This is a technique established on the basis of immunology, biochemistry and microscopy techniques, which uses antigen-antibody reactions to locate antigenic substances in tissues or cells.
Fluorescence analyzer is an instrument that uses the fluorescence effect of fluorescent substances for detection. There is fluorescein on the immunochromatography test strip, and after being irradiated by the excitation light source and absorbing incident light of a certain wavelength, it can emit Fluorescence with a slightly longer wavelength, when the light source stops irradiating, the emitted light also disappears. Fluorescein is used as a label to bind to known antibodies without affecting its immunological properties, and then the fluorescein-labeled antibody As a standard reagent, it is used to detect and identify unknown antigens. After the immunoreaction between the antigen and the antibody, the fluorescein bound to the antibody is released. The more antigens that are immunoreacted with the antibody, the more fluorescein is released. Fluorescence The more fluorescence is produced by the effect, on the contrary, the less fluorescence is produced. By converting the fluorescence intensity into an electrical signal, the content of the corresponding antigen in the detected substance can be known by analyzing the electrical signal.
