The background is wrong when counting. Set the automatic detection of blank value during cleaning. If the detection blank exceeds the specified value, an alarm will be issued, and you can know that a background error has occurred. First check whether the diluent or hemolytic agent has air bubbles and is contaminated and if so, replace the reagent. In addition, it is necessary to check whether it is interfered with by electromagnetic waves and ensure that the grounding is good. In addition, if the pores of the detector in the detection room are contaminated, it will also cause the fault. You can start the maintenance cleaning program for cleaning. If none of the above causes, you can check whether the rotary valve is contaminated and deal with it accordingly.
2. RBC/WBC/PLT counting error. You can first check whether it is caused by sample agglutination, and replace the sample if it is. If it is not the reason for the sample, check whether it is interfered with by noise, electromagnetic waves, whether the grounding is faulty, etc. In addition, abnormal emptying of the insulation chamber below the RBC, WBC, and HGB detection chambers can also cause this fault and should be eliminated. Check whether the detection chamber or the pores of the detector are contaminated, whether the pores of the detector are damaged, whether the pipeline between the cell counting quantitative diaphragm pump and the detector is blocked, etc. In addition, it is necessary to eliminate the circuit board failure
In order to ensure that the results obtained by using the blood cell analyzer can reflect the true condition of the patient as much as possible, please pay attention to: 1. Blood sample: Because the venous blood is less affected by external factors, the composition is relatively stable, and the test results are highly accurate and reproducible. Therefore, except for infants, venous blood should be used for all blood collections. If collecting the final blood, be careful not to over-squeeze it locally to avoid mixing a large amount of tissue fluid in the blood, and it is easy to activate the coagulation system to produce local coagulation, leading to errors in the test results; the first drop of blood should be discarded due to unstable cell components. Test with the second drop of blood. 2. Anticoagulant: using catenate anticoagulant for too long is easy to crystallize, cell morphology is easy to change, which affects the accuracy of counting results; oxalate is easy to cause platelet aggregation, and can change the morphology of white blood cells, affecting Counting results and classification; excessive heparin anticoagulation can easily cause leukocyte aggregation and thrombocytopenia; EDTA-2Na is less soluble than EDTA-2K, and platelet aggregation is more likely. Therefore, the International Committee for Hematology (ICSH) in 1993 recommended the use of EDTA-2K as an anticoagulant for blood cell analyzers, with a dosage of 1.5-2.2 mg/ml blood. 3. Seal the blood with a stopper and store at room temperature for no more than 6 hours. 4. Dilution: The diluter and suction tube must be calibrated. After sucking blood, the blood outside the suction tube should be wiped clean completely. The blood should be measured as soon as possible after dilution, otherwise it is easy to cause "dilution hemolysis". 5. Mixing: Mixing is very important before testing. If there is no rotating mixer, it should be inverted and mixed at least 8 times. 6. Reagents: The blood cell analyzer has very strict requirements for reagents, requiring strict osmotic pressure standards, stable electrical conductivity, high standards of purity, and non-corrosive effects on instrument pipelines and valve circuits. Therefore, the hemolytic agent, diluent and cleaning agent, etc. are best to use the original accessory products. 7. White blood cell classification: First of all, it must be clear that so far, no matter how advanced blood cell analyzers in the world, white blood cell classification is only a screening method and cannot completely replace manual microscopic classification. We must resolutely correct the wrong idea that some units use blood cell analyzers to discard microscopic examinations. 8. Quality control: A strict quality control system must be established for blood analysis to ensure the reliability of the results.
