That is, the enzyme-linked immunosorbent assay is a special instrument for enzyme-linked immunosorbent assay, also known as a microplate detector. It can be simply divided into two categories: semi-automatic and fully automatic, but their working principles are basically the same. The core is a colorimeter, that is, colorimetric analysis is used. The determination generally requires the final volume of the test solution to be less than 250μL, and the test cannot be completed with a general photoelectric colorimeter, so there are special requirements for the photoelectric colorimeter in the microplate reader.
principle
The microplate reader is actually a phase-changing photoelectric colorimeter or spectrophotometer, and its basic working principle is basically the same as the main structure and photoelectric colorimeter. The figure shows the working principle diagram of a single-channel automatic sampling microplate reader The light wave emitted by the light source lamp is transformed into a monochromatic light by the filter or monochromator, and enters the specimen under test in the plastic microporous pole. Part of the monochromatic light is absorbed by the specimen, and the other part is irradiated through the specimen. On the photodetector, the photodetector converts the different light signals of the specimen to be tested into corresponding electrical signals. The electrical signals are processed by pre-amplification, logarithmic amplification, and analog-to-digital conversion, and then sent to micro-processing The processor performs data processing and calculation, and finally displays the results by the display and printer. The microprocessor also controls the movement of the mechanical drive mechanism in the X direction and Y direction to move the microplate through the control circuit, so as to realize the automatic sampling detection process. The microplate reader is used to manually move the microplate for detection, so the mechanical drive mechanism and control circuit in the X and Y directions are omitted, so that the instrument is smaller and the structure is simpler.
The microplate reader is actually a phase-changing photoelectric colorimeter or spectrophotometer, and its basic working principle is basically the same as the main structure and photoelectric colorimeter. The figure shows the working principle diagram of a single-channel automatic sampling microplate reader The light wave emitted by the light source lamp is transformed into a monochromatic light by the filter or monochromator, and enters the specimen under test in the plastic microporous pole. Part of the monochromatic light is absorbed by the specimen, and the other part is irradiated through the specimen. On the photodetector, the photodetector converts the different light signals of the specimen to be tested into corresponding electrical signals. The electrical signals are processed by pre-amplification, logarithmic amplification, and analog-to-digital conversion, and then sent to micro-processing The processor performs data processing and calculation, and finally displays the results by the display and printer. The microprocessor also controls the movement of the mechanical drive mechanism in the X direction and Y direction to move the microplate through the control circuit, so as to realize the automatic sampling detection process. The microplate reader is used to manually move the microplate for detection, so the mechanical drive mechanism and control circuit in the X and Y directions are omitted, so that the instrument is smaller and the structure is simpler.
use
It can be widely used in low-ultraviolet region DNA, RNA quantification and purity analysis (A260/A280) and protein quantification (A280/BCA/Braford/Lowry), enzyme activity, enzyme kinetic detection, enzyme-linked immunoassays (ELISAs), cell Proliferation and toxicity analysis, cell apoptosis detection (MTT), reporter gene detection and G protein coupled receptor analysis (GPCR), etc.
