What is the principle of magnetic bead nucleic acid extractor?
Nucleic acid extraction machine classification Nucleic acid extraction instrument is an instrument that uses matching nucleic acid extraction reagents to automatically complete sample nucleic acid extraction.
Nucleic acid extraction machines can be divided into two categories: one is large-scale automation, commonly known as automatic liquid workstation; the other is large-scale automated liquid workstation; the other is small nucleic acid automatic extractor, which uses packaged reagents to automatically complete the extraction and purification process. Large-scale automatic liquid workstations are rarely used due to high equipment and operating costs, and are suitable for extracting thousands of similar samples at a time; small automatic instruments are widely used because of their low cost and convenient operation.
Application of nucleic acid extraction machine
In almost all laboratories, the separation and purification of biomolecules is very important and necessary. However, purifying multiple samples is quite difficult. It is not only necessary to select a suitable purification process, but also the workload is particularly large, which is difficult to meet the needs of the rapid development of high-throughput sample extraction and purification.
Nucleic acid extractor The nucleic acid extractor is an instrument that uses the supporting nucleic acid extraction reagents to automatically perform nucleic acid extraction from samples. It has been widely used in environmental microbial testing, food safety testing, blood transfusion safety, forensic identification, disease control centers, clinical disease diagnosis, biological research and animal husbandry, etc.
1. The separation of nucleic acid by magnetic rod method is realized by the transfer of magnetic beads and the immobilization of liquid. The principle and process are the same as the suction method. Magnetic beads are separated from liquids differently. The magnetic rod method uses a magnetic rod to adsorb magnetic beads, separate the magnetic beads from the waste liquid, and put them into the waste liquid in the next step to achieve nucleic acid extraction.
2. Suction method Suction method, also known as pipetting method, uses liquid transfer or magnetic bead fixation to achieve nucleic acid extraction. Usually, the transfer is achieved by the operation of the system controlled manipulator, including the following extraction process:
1. Pyrolysis: Add the pyrolysis solution to the sample, mix the reaction solution, and complete the reaction through mechanical movement and heating. Cut cells, release nucleic acids
2. Adsorption: Add the magnetic beads to the sample lysate, mix well, and use the magnetic beads to adsorb nucleic acids because they have a strong affinity for nucleic acids under high salt and low pH conditions. Under the action of the external magnetic field, the magnetic beads are separated from the solution, and the magnetic beads are transferred to the waste liquid tank through the suction tip, and the suction tip is discarded
3. Cleaning: remove the external magnetic field, add the cleaning buffer of the new tip, stir evenly, remove impurities, and remove the liquid under the action of the external magnetic field
4. Elution: remove the external magnetic field, add elution buffer with a new tip, mix well, and separate the bound nucleic acid from the magnetic beads to obtain purified nucleic acid
(1) The nucleic acid extractor should be at least 10 cm away from other vertical surfaces
(2) The input power cord of the instrument must be grounded to prevent electric shock
(3) The operator shall not disassemble the instrument without authorization. Component replacement or internal adjustments must be performed by a licensed professional service technician. Do not replace parts while the power is on
(4) The relative humidity is 10%-80%, the air flows smoothly at 35°C or 40°C lower than the normal atmospheric pressure; the altitude should be lower than 3000 meters, the temperature is 20-35°C, and the typical working temperature is 25°C
(5) Do not place the instrument near heat sources, such as electric heaters. Prevent water or other liquids from splashing into electronic components to avoid short circuits
(6) The air inlet and exhaust port are located on the back of the instrument. At the same time, dust or fibers should be prevented from accumulating in the air intake, and the air duct should be kept clear.
