From blood vessels to biochemical analyzers, precautions for blood ammonia detection

From blood vessels to biochemical analyzers, precautions for blood ammonia detection

Patient preparation：

1. No smoking: smoking should be prohibited after midnight the day before blood ammonia test specimens are collected;

2. Diet: Too much high-protein food before blood sampling can cause blood ammonia concentration to rise;

3. Drugs: Cefoxitin and Intolipid injection at therapeutic levels can increase and decrease the blood ammonia concentration;

4. Exercise: Long-term high-intensity exercise will cause the blood ammonia concentration to rise.

Sample collection：

1. Blood collection tube: a test tube made of de-ammonia material should be selected;

2. Anticoagulant: Non-ammonium salt of heparin and EDTA anticoagulant should be used for anticoagulant samples, and sodium citrate, sodium fluoride, and ammonium heparin should not be used for anticoagulation;

3. Collection time: specimens should be collected early in the morning on an empty stomach;

4. Blood collection site: It is recommended to collect venous blood;

5. The operation should be standardized during specimen collection, and the mixing action should be gentle and gentle to avoid hemolysis of the specimen, which may lead to a false increase in blood ammonia.

Sample shipment：

After the specimen is collected, the collection time should be marked, and the specimen should be placed vertically in ice water and sent for inspection immediately to avoid violent shaking.

Sample testing：

1. When adding samples to the reagent tray, only add blood samples, not diluents;

2. It is recommended to use fresh plasma or whole blood for the sample, not serum;

3. After sampling, the sample should be placed at 4°C for 30 minutes to complete the test;

4. If the determination cannot be made in time, the plasma should be separated and stored at low temperature and protected from light. The sample test does not need to return to normal temperature. The test should be completed within 2 hours when stored at 4°C; the test should be completed within 24 hours when stored at -20°C.

Specimen status：

1. Hemolysis: The blood ammonia concentration in red blood cells is higher than that in plasma, and hemolysis of the sample will lead to an increase in blood ammonia concentration;

2. Hyperbilirubinemia and Lipidemia: The high concentration of bilirubin and lipid turbidity in the plasma will interfere with the enzymatic determination of blood ammonia and affect the accuracy of the test results.

environmental impact：

Ammonia in the laboratory air is the cause of ammonia contamination of specimens. In order to reduce the contamination of specimens and utensils by ammonia in the air, the quality of the air in the laboratory should be guaranteed. The hospital should strictly ban smoking, restrict personnel from entering and leaving the laboratory, and test utensils must be used before they are used. After special cleaning.

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