Principle of high pressure steam sterilizer

Jun 24, 2021 Leave a message

Principle of high pressure steam sterilizer

The principle of high pressure steam sterilizer is:

in a closed steamer, the steam cannot overflow, and the pressure continues to rise, so that the boiling point of the water continues to increase, so that the temperature in the pot also increases. Under a pressure of 0.1MPa, the temperature in the pot reaches 121°C. At this steam temperature, it can quickly kill all kinds of bacteria and its principle of high heat resistance. To achieve the purpose of sterilizing moisture-resistant and high-temperature resistant items.

 

Precautions:

Completely remove the air in the pot so that all the pot is steam, and the sterilization can be complete.

 

After reaching the holding time, the power supply can be cut off, and care should be taken not to reduce the pressure too fast, so as to cause violent decompression boiling and overflow the liquid in the container.

 

For items that do not deteriorate after autoclaving, such as sterile water, cultivation media, and inoculation equipment, the sterilization time can be extended or the pressure can be increased. The medium must strictly abide by the holding time. It is necessary to maintain the pressure thoroughly and prevent the ingredients in the medium from deteriorating or reducing the effectiveness. The time cannot be extended arbitrarily.

 

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