PCR Nucleic acid extraction instrument types, advantages
Application fields of nucleic acid extractor: In almost every laboratory, the separation and purification work related to biomolecules is very important and indispensable. However, it is quite difficult to purify multiple samples, not only need to select the appropriate purification technology, but also the workload is very large, it is difficult to meet the current rapid development of high-throughput sample extraction and purification needs.
Nucleic acid extractors are mainly used in nucleic acid extraction, nucleic acid protein extraction and separation. However, when purchasing a nucleic acid extractor like this comparison, you must pay attention to a few points. Here are some points for users. Suggestion for purchase: There are already many small automatic nucleic acid extractors, and there are also many domestic companies producing them. However, very few are actually used. Before purchasing, customers should follow the following principles:
1. A prototype must be provided
Carry out the actual operation of the prototype, check the operation process and test results. Although most of them are advertised as automatic extraction, they require a lot of manual operations, and the extraction effect does not necessarily meet the requirements of their next experiments.
2. Agree on the price of extraction reagents
When signing the contract, the price and brand of the extraction reagent must be agreed in the contract at the same time, because most of the extraction reagents are expensive, not the nominal price of the sales promoter during the test.
What are the types of our common nucleic acid extractors?
Nucleic acid extractor has two classification methods, one is to classify according to use, the other is to classify according to different application reagents.
(1) Generally, it can be divided into two categories according to the purpose: one is the automatic liquid workstation, which is a very powerful device, which can automatically complete liquid dispensing and suction, and can even integrate expansion, detection and other instruments and equipment. Complete automation of sample extraction, amplification, and detection. Nucleic acid extraction is only an application of its function, and it is not suitable for routine laboratory extraction of nucleic acid. It is generally used in the experimental needs of a single type of specimen and a very large amount of specimens (at least 96, usually several hundred) extracted at one time. The establishment of the platform of the automatic workstation and the operation of the platform require relatively large funds.
The other type is small automated instruments. Small automated instruments achieve the purpose of automatically extracting nucleic acids through the special design of the operating structure, and can be applied in any laboratory. In recent years, many models have been put on the market one after another.
(2) According to the different reagents used, it is divided into two categories: one is the instrument using the magnetic bead method reagent, and the other is the instrument using the spin column method reagent. The automatic nucleic acid extractor using the magnetic bead method is easy to operate and easy to automate, and is currently the mainstream in the market.
What are the advantages and features of the nucleic acid extractor compared with traditional manual operations?
Nucleic acid extraction instruments generally have the following characteristics:
(1) No cross-contamination: the instrument parts are not in contact with biological samples;
(2) Automated system: for each extraction, the operator only needs to do the installation work and arrange the well plate (provide the reagents required for extraction). The whole extraction process does not require human help, and the instrument completes automatically;
(3) Extraction control: In the extraction process, if the operator needs, the system allows you to control the extraction process.
