Urine Analyzer
Urine analyzer is an automated instrument for determining certain chemical components in urine. It is an important tool for automated urine inspection in medical laboratories. It has the advantages of simple and fast operation. Under the control of the computer, the instrument collects and analyzes the color information of various reagent blocks on the test strip, and undergoes a series of signal conversion, and finally outputs the measured chemical composition content in the urine.
working principle
The essence of the test principle of the urine analyzer is the absorption and reflection of light. The liquid sample is directly added to the multi-linked reagent strip with different solidified reagents. The corresponding chemical composition in the urine causes the color of the module containing various special reagents on the multi-linked reagent strip to change. The color depth is consistent with the specific chemistry in the urine sample. The component concentration is proportional; the multi-link test strip is placed in the colorimetric injection tank of the urine analyzer, and each module is irradiated by the light source of the instrument and produces different reflected light. The instrument receives the light signal of different intensity and converts it into the corresponding The electrical signal is calculated by the microprocessor (CPU) to calculate the reflectance of each test item, and then compared with the standard curve and corrected to the measured value, and finally the result is automatically printed out in a qualitative or semi-quantitative manner.
This type of instrument is generally controlled by a computer, and the color change on the test strip is measured semi-quantitatively by using a spherical area spectrometer to receive dual-wavelength reflected light. There are several reagent pads containing various reagents on the reagent strip, each of which reacts independently with the corresponding components in the urine, and displays different colors. The depth of the color is proportional to a certain component in the urine. There is another one in the reagent strip. "Compensation pad", as the background color of urine, compensates for errors caused by colored urine and instrument changes.
The instrument usually calculates the reflectance automatically according to the following formula, and then compares with the standard curve, and automatically finds the corresponding results of various components. If the content of a certain component in urine is high, the reflection light of the corresponding reagent pad is darker, otherwise it is strong .
Reflectance fraction: R(%)=Tm.Cs/TsCm×100%
In the formula, R (%) is the reflectivity; Tm is the reflection intensity of the reagent pad to the measurement wavelength; Ts is the reflection intensity of the reagent pad to the reference wavelength; Cm is the reflection intensity of the calibration pad to the measurement defect length; Cs is the calibration pair The intensity of the reflection at the reference wavelength.
