1. The principle of wet chemical method is based on the Lambert-Beer law. The reaction carrier is an aqueous solution. The incident light is absorbed by the colored reaction product and then weakened. The concentration of the measured substance is reflected by the absorbance.
Dry chemistry, also known as solid-phase chemistry, uses solid-phase reagent technology with multilayer thin films. As long as the liquid sample is directly added to a reagent carrier that has been solidified in a special structure, that is, a dry chemical reagent, the water in the sample is used as a solvent to dissolve the reagent solidified on the carrier, and then mixed with the component to be tested in the sample. A chemical reaction is carried out to carry out analysis and determination, and finally the concentration or activity of the object to be measured is obtained.
dry chemistry vs wet chemistry
dry chemistry | wet chemistry | |
Detection method | reflection photometry | Transmittance photometry |
reaction vector | solid medium | Cuvette or flow cell |
piping system | No liquid pipeline | with liquid pipeline |
theoretical basis | Kubelka Munk | Lambert Beer |
maintain | simpler | More |
reagent | For solid phase, most of them do not need calibration, and the stability period is long; for liquid, calibration is required, and the stability period is short. | |
2. Advantages of dry chemistry
High accuracy
Sensitivity and accuracy are similar to typical discrete instruments.
Fast detection
Multilayer film technology, instant measurement after sample addition, short detection time
Wide range of applications
Flexible usage scenarios, suitable for laboratory departments, blood banks in clinical departments, primary medical and health institutions, physical examination centers, etc.
Environmentally friendly and pollution-free
No need to wash, clean and environmentally friendly waste only reagent cards, easy to collect and dispose of
Small and lightweight
The instrument is simple in structure, small in size and light in weight
easy to use
No liquid pipeline
Storage at room temperature, no need to rewarm, long-term stability without electrode maintenance, no need to replace the electrode membrane
Influencing factors of dry chemical analysis technology
1. Quality control substances
2. Storage and use of dry tablet reagents
3. Working environment and temperature
