Q: Do I need to buy a biological safety cabinet or a clean bench
A: The ultra-clean workbench provides protection for the samples, but does not provide protection for the operator and the environment; in a broad sense, the biological safety cabinet provides protection for the operator, the environment and the sample at the same time. Therefore, from the protection situation you need, you can draw the right choice;
Q: Which type and protection level of biological safety cabinet should I buy
A: Generally speaking, unless you need to do some unknown strains, viruses and living organisms, you need to use a three-level biological safety cabinet, and the two-level biological safety cabinet can basically meet our normal work needs. For the experiment of samples that do not contain toxic, radioactive, volatile and irritating solvents, the A1/A2 (formerly B3) type biological safety cabinet has already met the requirements for use. For the procurement of the hospital system, because the types of viruses and bacteria are more complicated, and the experimental conditions Great changes, it is recommended to use B2 full row biological safety cabinet;
Q: How to judge the quality of a biological safety cabinet
A: The biological safety cabinet is mainly used for biohazard protection, so the protection of the operator is the main basis for judging the quality of the product. The core technology of the secondary biological safety cabinet is the filter membrane (including filter membrane material, filter membrane retention efficiency, filter membrane life), air path design (including the balance of the front operating surface, the settling wind speed and the uniformity of the wind speed, and the inlet wind speed , Vertical settlement of wind), alarm settings (a good biosafety cabinet should include low wind speed alarms, alarms for the wrong position of the front window, UV interlocking device, B2 full row models also need to have an external fan interlocking device) and negative pressure protection (Including side wall negative pressure, back negative pressure, top negative pressure). Therefore, the advantages and disadvantages of the biological safety cabinet can be known through the comparison of the above indicators;
Q: Why is the balance of the front operating surface an important indicator of a biological safety cabinet
A: All secondary biological safety cabinets have an open front operating surface, which is easy to operate and also brings a possibility of pollution. If the balance is not good, then this area will be polluted, and it will not be possible to prevent the outside from entering normally. The contamination of samples in the operation room and the protection of the operator outside the operation room will cause an essential deviation from the design concept of the biological safety cabinet. Therefore, the balance of the front operation surface of the secondary biological safety cabinet is critical;
Q: Why is there a low wind speed alarm in the alarm device?
A: In Europe and America, according to European and American standards, it is recommended to check the main technical indicators for every biosafety cabinet. After we buy the biological safety cabinet and use it for a period of time, whether the biological safety cabinet is still in the normal protection of the operator, the environment and the sample, as the operator, we have no way to know, and once the sedimentation is caused by the accumulation of the filter membrane If the wind speed decreases, sample contamination may occur. If the inlet wind speed decreases, then personnel may be contaminated. Under the current national conditions, it is difficult for us to perform annual technical inspections, because there is a large cost, so low wind speed alarms are very important;
Q: The biological safety cabinet has an electric front window, is it very convenient and good?
A: Wrong! In Europe and America, a biohazard laboratory often needs to be equipped with a dual power supply system to avoid leakage of biohazardous substances when a power supply system fails. In China, such a laboratory configuration is difficult to obtain. Therefore, once the power of the electric front window is cut off during the experiment, it will cause great danger and cause irreparable losses! Moreover, whether it is an electric front window or a manual front window, the height is fixed during operation. Therefore, on the biological safety cabinet, the electric front window is of little significance and dangerous, and it can be said that all harms are not beneficial;
Q: What does the negative pressure on the side wall of the biological safety cabinet mean?
A: The European and American standards for biological safety cabinets are different, and the requirements on the side walls are also different. American products are all stainless steel side walls, and a negative pressure cavity is required to avoid the possibility of side wall leakage and pollution. European products basically use glass side walls, and there is no opposite side wall. Do negative pressure protection. Since the operating room inside the cabinet is under positive pressure, if there are shell damage and leakage points, external leakage will occur. Therefore, the formation of negative pressure protection on the side wall and back plate can effectively reduce the possibility of leakage;
Q: How to avoid contamination and cross-contamination in the negative pressure protection cavity
A: With a good design, the fan can be idle for a period of time to clean and disinfect after the end of the work. Without this design, the operator needs to continue to open the cabinet for a certain period of time after the end of the experimental operation to maximize the discharge of pollutants.
Q: What accessories do we need to order?
A: Generally, some electrical equipment is needed in the cabinet, so the splash-proof power socket in the cabinet is needed. And because the European instruments are more popular in my country, the grounding of the European power cord is incompatible with the sockets in our country, so A certain amount of static electricity will be generated on European-style instruments. Static electricity will affect the vertical settlement of the wind flow in the biological safety cabinet, and will have a certain adsorption effect. Therefore, if possible, it is best to configure a European-style splash-proof power socket. Since the pollution inside the operation room of the cabinet is not very large, and the biggest pollution is in the negative pressure protection cavity, the UV lamp system can be selected to achieve better sterilization and disinfection functions. A safety cabinet with a split design needs to be equipped with a bracket;
In addition to understanding the performance indicators of the safety cabinet, the best, fastest and most convenient way to identify the safety performance of the safety cabinet is to know whether the safety cabinet you purchased has obtained the following major international safety cabinet certifications:
EN12469 (Unified European Standard for Biological Safety Cabinets)
NSF49 (U.S. Standard for Biological Safety Cabinets-Secondary Biological Safety Cabinets)
AS2252.2 (Australian Standard for Biological Safety Cabinet-Class II Biological Safety Cabinet) JIS K3800 (Standard for Japanese Biological Safety Cabinet)
While seeking the manufacturer’s product certification, users may pay attention to the following:
1. When the manufacturer claims that the safety cabinet produced by itself conforms to a certain international standard, it can be required to produce a certificate-an independent test report signed by a legal certification body;
2. Some standards may not be directly related to the safety performance of the safety cabinet. For example, the manufacturer of the biological safety cabinet claims to have obtained ISO9001 quality certification, and ISO9001 does not perform the above-mentioned international safety cabinet standard testing on the performance of the safety cabinet;
3. The certification must be tested and promulgated by an independent and legal safety cabinet certification body;
